FAQs & Forms

Sample Submission – Frequently Asked Questions

General Guidelines

> Ensure maximum 48 hours transit time during the hottest months.

> Do not ship before holidays / long weekends.

> Proper packaging is essential for safety and compliance – follow all regulations for labeling and transport of diagnostic specimens.

> Reference Fedex Guide: https://www.fedex.com/content/dam/fedex/us-united-states/services/UN3373_fxcom.pdf.

> We recommend not using the United States Postal Service (USPS) for diagnostic shipments. Even if shipped express, USPS deliveries often take an additional 2 days. For faster turnaround, use FedEx or UPS.

Packaging Requirements

> Primary Container: Use a watertight, leakproof container (e.g., 10 mL ear notch tube, red/yellow top tube, or screw-cap tube). Label each sample with animal ID.

> Secondary Packaging: Wrap each primary container in absorbent material (e.g., paper towel) and place in a leakproof zip-seal bag. Separate multiple tubes in a single ziplock bag with absorbent material.

> Outer Packaging: Use sturdy cardboard box (pregnancy samples) or insulated cooler (disease samples). Include ice packs (not frozen water bags) that are seperated from the secondary pacakaging by paper towels or bubble wrap. Must withstand a 4-foot drop test.

> Submission Form: Place in waterproof bag. Ensure contact details (address, phone, email) are included.

> Labeling: Mark as “Exempt Animal Specimen” on the outer packaging.

Important Safety Reminders

> Do not send samples in syringes with needles attached (packages with sharps will be rejected).

> Do not use gloves, OB sleeves, or leaky containers for sample transport.

> Protect all samples from heat and freezing — hemolyzed or frozen samples may be rejected.

Pregnancy Testing

> Collect whole blood into red-top / yellow-top tubes (no anticoagulant).

> Allow tubes to clot upright at room temperature for 30–60 minutes before refrigeration.

> After clotting, refrigerate at 2–8 °C until shipping.

> Do not freeze.

Serological Biosecurity Testing with Serum

(Red/yellow top tubes for tests such as Johne’s ELISA, CAE/OPP, CL)

> Collect whole blood into red-top / yellow-top tubes (no anticoagulant).

> Allow tubes to clot upright at room temperature for 30–60 minutes before refrigeration.

> After clotting, refrigerate at 2–8 °C until shipping.

> Do not freeze.

Plasma Samples (Purple Top Tube)

> Collect whole blood into purple-top tube (EDTA anticoagulant).

> Invert gently to mix — do not shake.

> Keep refrigerated (2–8 °C) until shipping.

> Do not freeze.

Ear Notch Samples (e.g., BVDV Ag)

> Collect tissue in a 10 mL ear notch tube (preferred) or other sterile, leakproof tube.

> Keep refrigerated (2–8 °C) until shipping.

> Do not freeze.

Fecal Samples (e.g., Johne’s PCR)

> Must be fresh and collected directly from rectum (not ground).

> Submit in 10 mL ear notch tube (preferred), other leakproof sterile tube, or sealed plastic bag.

> Avoid overfilling to prevent leakage.

> Do not freeze.

> Ship overnight to lab as soon as possible to prevent degradation and egg hatching.

Lymph Node Abscess Samples (e.g., CL PCR)

> Collect purulent material from abscess using sterile syringe (no needle / syringe for shipping) or swab.

> Use sterile polyester (Dacron) or nylon flocked swabs with plastic shafts. Avoid cotton, calcium alginate, or wooden shaft swabs.

> Place in 10 mL ear notch tube (preferred) or other sterile, leakproof screw-cap tube.

> Ship overnight to lab to prevent nucleic acid degradation.

Swine Milk Samples (PEDV – IgA test)

> Collect into 10 mL ear notch tube (preferred) or other sterile, leakproof tube.

> Avoid manure/debris contamination.

> Keep refrigerated until ready to ship (2–8 °C).

Swine Blister Fluid Samples (SVA PCR)

> Collect aseptically from vesicles using sterile syringe (no needle for shipping) or swab.

> Place in 10 mL ear notch tube (preferred) or other sterile, leakproof tube.

> Keep refrigerated until ready to ship (2–8 °C).

> Ship overnight on ice packs.

Swine Oral Fluid Samples (e.g., PEDV PCR)

> Collect using rope-chew method or sterile swab.

> Transfer into 10 mL ear notch tube (preferred) or other sterile, leakproof tube.

> Use sterile polyester (Dacron) or nylon flocked swabs with plastic shafts. Avoid cotton, calcium alginate, or wooden shaft swabs.

> Keep refrigerated until ready to ship (2–8 °C).

> Ship overnight on ice packs.

Nasal Swabs (e.g., CDV PCR)

> Use sterile polyester (Dacron) or nylon flocked swabs with plastic shafts. Avoid cotton, calcium alginate, or wooden shaft swabs.

> Nasal Swab: Collect from both nostrils with sterile swab.

> Place in 10 mL ear notch tube (preferred) or other sterile, leakproof tube with VTM or saline.

> If not available, swabs may be submitted dry (reduced sensitivity).

> Keep refrigerated until ready to ship (2–8 °C).

> Ship overnight on ice packs.